Synonym(s): α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride
Empirical Formula (Hill Notation): C7H7FO2S
Molecular Weight: 174.19
| Cat nr | Stock | Quantity (gr) | Price |
| 20240608-10 | In stock | 10 g | €35 |
| 20240608-100 | In stock | 100 g | €240 |
| 20240608-1000 | In stock | 1 kg | €1300 |
**Pricing disclaimer
Application
Phenylmethanesulfonyl fluoride (PMSF) is widely used in molecular biology and biochemistry as a serine protease inhibitor. It is commonly incorporated in radioimmunoprecipitation assay (RIPA) buffer for western blot analysis to prevent protein degradation during sample preparation【source】 . PMSF is also used in the lysis buffer for RAW264.7 cells, a murine macrophage cell line, during western blotting to ensure the integrity of proteins during extraction【source】.
Biochemical and Physiological Actions
PMSF plays a crucial role in enhancing the stability of the plasma lipidome, which is important in lipidomic and metabolomic analyses【source】. Additionally, PMSF has been shown to reduce naloxone-precipitated withdrawal jumping behavior in morphine-dependent mice, highlighting its potential influence on certain physiological processes【source】. This indicates its broader application beyond a protease inhibitor, potentially affecting neurochemical pathways related to addiction and withdrawal【source】.
